منابع مشابه
Controlling the speed of hirudin folding.
The folding of hirudin undergoes an initial stage of non-specific packing, followed by consolidation (re-organization) of partially packed intermediates to attain the native structure [Chatrenet and Chang (1993) J. Biol. Chem. 268, 20988-20996]. Non-specific packing leads to the formation of scrambled hirudins as folding intermediates. A systematic study was carried out to search for conditions...
متن کاملOxidative folding of hirudin in human serum.
Human serum contains factors that promote oxidative folding of disulphide proteins. We demonstrate this here using hirudin as a model. Hirudin is a leech-derived thrombin-specific inhibitor containing 65 amino acids and three disulphide bonds. Oxidative folding of hirudin in human serum is shown to involve an initial phase of rapid disulphide formation (oxidation) to form the scrambled isomers ...
متن کاملThe disulfide folding pathway of hirudin elucidated by stop/go folding experiments.
The folding pathway of hirudin was analyzed by structural characterization and stop/go folding experiments of acid-trapped intermediates. The results show that the folding is initiated by a near-random packing, followed by the reorganization and fine adjustment of partially compact intermediates to attain the active molecule. The process of packing is observed as the unfolded hirudin flows sequ...
متن کاملThe protein folding 'speed limit'.
How fast can a protein possibly fold? This question has stimulated experimentalists to seek fast folding proteins and to engineer them to fold even faster. Proteins folding at or near the speed limit are prime candidates for all-atom molecular dynamics simulations. They may also have no free energy barrier, allowing the direct observation of intermediate structures on the pathways from the unfo...
متن کاملExternally Adaptive Speed Controlling
As the volume of traffic on highways and roadways continues to grow at a greater rate than our roads can accommodate the effect of traffic congestion will become an ever increasing burden on city infrastructure. One solution is an adaptive system using real-time signal control to optimize signal timings and minimize delays. A large number and variety of ATCS(Adaptive Traffic Control Systems) ha...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1994
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj3000643